T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA. T4 DNA Ligase joins blunt end and cohesive end termini as well as repairs single stranded nicks in duplex DNA and some DNA/RNA hybrids (1).
Immobilized T4 DNA Ligase (IM T4 DNA Ligase) is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, enzyme can be removed using a magnet, and can be re-used.
Temperature |
% Activity |
---|---|
16ºC |
100% |
25ºC |
100% |
37ºC |
50% |
Buffer |
pH |
% Activity |
---|---|---|
NEBuffer r1.1 |
7.0 |
50% |
NEBuffer r 2.1 |
7.9 |
100% |
NEBuffer r3.1 |
7.9 |
70% |
rCutSmart Buffer |
7.9 |
100% |
Phi29 DNA Polymerase Buffer |
7.5 |
100% |
Standard Taq Buffer |
8.3 |
70% |
Thermopol Buffer |
8.8 |
70% |
Q5 Reaction Buffer |
8.5 |
10% |
T4 DNA Ligase Buffer |
7.5 |
100% |
NEBNext Quick Ligation Buffer |
7.6 |
25% |
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