Christine Sumner:
NEB offers two NEB next library prep kits for ion torrent. The fast DNA library preps that and the fast DNA fragmentation in library preps that. Both kits provide a fast streamlined workflow for inputs as low as 10 nanograms. Both kits contain reagents for and repair adapter ligation and PCR amplification. Only one of these kits provides reagents for DNA fragmentation. If you want to shear your DNA by an enzyme based fragmentation method, choose the fast DNA fragmentation library prep set for ion torrent catalog number E6285 this kit includes fragmentase, an enzyme mix that randomly fragments DNA. The randomness of cleavage of fragmentase is similar to mechanical sharing methods. The fragmentase in this kit has been optimized for use with the ion torrent protocol.
The size of the DNA fragments is controlled by incubation time. Sharing with fragmentase is easily scalable and does not damage the DNA. If you are using Kovars or other mechanical shearing methods or you are using chip DNA, the fast DNA library preps that catalog number E6270 is the right choice. The components and reaction volumes of this kit had been optimized for input DNA that has already been fragmented. This kit does not include fragmentation reagents. For more details, check out the ion torrent pages at NEBnext.com.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.